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Macconkey agar

Macconkey agar

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1V846 140.500

 

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Référence 105465
Synonymes MACCONKEY agar
Valeur de pH 6.9 - 7.3 (50 g/l, H₂O, 25 °C) (après autoclavage)
Densité 720 kg/m3
Solubilité 50 g/l
Classe de stockage 10 - 13 Autres liquides ou matières solides
WGK WGK 1 pollue faiblement l'eau
Élimination 3
Les réactifs organiques liquides relativement stables du point de vue chimique sont rassemblés dans le récipient A. S'ils contiennent des halogènes, les mettre dans le récipient B. Résidus solides: récipient C.
Appearance (clearness) clear
Appearance (colour) dark red
pH-value (25?°C) 6.9 - 7.3
Typical composition (g/litre): Peptone from gelatine 17.0; Peptone from casein 1.5; Peptone from meat 1.5; Sodium chloride 5.0; Lactose 10.0, Bile salt mixture 1.5; Neutralred 0.03; Crystal violet 0.001; Agar-Agar 13.5.
Growth promotion test in accordance with the harmonised method of EP, USP and JP.
Inoculum on reference medium (Escherichia coli ATCC 8739 (WDCM 00012)) 10 - 100
Inoculum on reference medium (Salmonella typhimurium ATCC 14028 (WDCM 00031)) 10 - 100
Inoculum on reference medium (Salmonella dublin ATCC 15480) 10 - 100
Inoculum on reference medium (Shigella sonnei ATCC 11060) 10 - 100
Inoculum on reference medium (Proteus mirabilis ATCC 29906 (WDCM 00023)) 10 - 100
Inoculum (Bacillus cereus ATCC 11778 (WDCM 00001)) > 10E+04
Inoculum (Staphylococcus aureus ATCC 6538 (WDCM 00032)) > 10E+04
Inoculum (Enterococcus hirae ATCC 8043 (WDCM 00089)) > 10E+04
Inoculum (Enterococcus faecalis ATCC 19433 (WDCM 00009)) > 10E+04
Inoculum (Enterococcus faecalis ATCC 29212 (WDCM 00087)) > 10E+04
Colony count (Escherichia coli ATCC 8739 (WDCM 00012))
Colony count (Salmonella typhimurium ATCC 14028 (WDCM 00031))
Colony count (Salmonella dublin ATCC 15480)
Colony count (Shigella sonnei ATCC 11060)
Colony count (Proteus mirabilis ATCC 29906 (WDCM 00023))
Recovery on test medium (Escherichia coli ATCC 8739 (WDCM 00012)) ? 50 %
Recovery on test medium (Salmonella typhimurium ATCC 14028 (WDCM 00031)) ? 30 %
Recovery on test medium (Salmonella dublin ATCC 15480) ? 30 %
Recovery on test medium (Shigella sonnei ATCC 11060) ? 30 %
Recovery on test medium (Proteus mirabilis ATCC 29906 (WDCM 00023)) ? 30 %
Colony colour (Escherichia coli ATCC 8739 (WDCM 00012)) red
Colony colour (Salmonella typhimurium ATCC 14028 (WDCM 00031)) colourless
Colony colour (Salmonella dublin ATCC 15480) colourless
Colony colour (Shigella sonnei ATCC 11060) colourless
Colony colour (Proteus mirabilis ATCC 29906 (WDCM 00023)) colourless
Medium colour (Escherichia coli ATCC 8739 (WDCM 00012)) red
Medium colour (Salmonella typhimurium ATCC 14028 (WDCM 00031)) yellowish
Medium colour (Salmonella dublin ATCC 15480) yellowish
Medium colour (Shigella sonnei ATCC 11060) yellowish
Medium colour (Proteus mirabilis ATCC 29906 (WDCM 00023)) yellowish
Precipitate (Escherichia coli ATCC 8739 (WDCM 00012)) +
Growth (Bacillus cereus ATCC 11778 (WDCM 00001)) none
Growth (Staphylococcus aureus ATCC 6538 (WDCM 00032)) none
Growth (Enterococcus hirae ATCC 8043 (WDCM 00089)) none
Growth (Enterococcus faecalis ATCC 19433 (WDCM 00009)) none
Growth (Enterococcus faecalis ATCC 29212 (WDCM 00087)) none
Incubation: 18 hrs.; 30-35°C; Gram-positive strains 24 hrs.
The information above is current at this time of publication and is subject to change without notice (except for customers holding a change control agreement with our company). The information/format can be altered at any time and inany way if internal company matters or Standard Regulationsdo say so.

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